Fluorescence microscopes are essential tools for visualizing labeled cells, proteins, and structures with high specificity. Whether you're analyzing fixed tissue slides or live-cell cultures, this guide helps you choose between upright and inverted systems, compare optics and light sources, and understand automation needs. Learn how to match microscope features like filter cubes, cameras, and software with your imaging workflow; and explore trusted models like the Olympus IX83 and Nikon Ti2.