Molecular Devices SpectraMax Gemini XS
Manufacturer: Molecular Devices
Model: SpectraMax Gemini XS
Status: Pre-owned
Condition: Very Good
Estimated lead time: 1-2 weeks
Guarantee: 15 day money back guarantee.
The SPECTRAmax GEMINI XS Microplate Spectrofluorometer is a microplate reader capable of detecting signal from either fluorescence or luminescence based assays. This device has all the features of a standard microplate reader, such as temperature control and shaker.
The most important feature of the SPECTRAmax GEMINI XS is that it uses two monochromators, instead of filters, to select the desired wavelengths. This allows the reader to be set in 1-nm increments, as opposed to the 25-nm increments typical of filters, over an excitation/emission range of 250 to 850 nm. The bandwidth for both the excitation and emission monochromators is 9 nm. The combination of setting the best possible wavelength and having a relatively tight bandwidth results in good sensitivity and specificity for any given probe. This also means that you can always choose the best excitation/emission wavelength pair for the fluorochrome of your choice. It is worth noting that the SPECTRAmax GEMINI XS can read both luminescence and fluorescence, the latter as an end-point, in a time-resolved fashion, or by scanning the spectra.
The fact that this instrument can be used for both fluorescence and luminescence based assays means that it is very versatile. This feature, as well as the design for multi-well plate tray, makes the SPECTRAmax GEMINI XS a valuable tool for a wide variety of applications: from ELISA to enzymatic assays, from reporter-gene activity measurements to cell survival/death assays. It also has a user-friendly interface – most of the settings can be changed by clicking on the intuitive icons on the machines screen.
Applications:
• Live/Dead viability/cytotoxicity assays
• Detection of nucleic acids
• Green fluorescent protein
• NanoOrange protein quantitation
• PicoGreen DNA detection
• Molecular beacons
• Caspase-3
• Fluorometric protease assays
• cAMP detection
Other similar items available: Spectramax M2e, Spectramax 190, Spectramax Plus 384
Fluorescence photometric performance
Dual monochromators (EX and EM) | 1 nm increment selection |
EX Wavelength range |
250-850 nm |
EM wavelength range |
360-850 nm |
Bandwidth (EX and EM) |
9 nm |
Detection limit (signal 3X SD of baseline) |
3.0 fmol/well FITC 200ul in 96 wells |
Time-resolved fluorescence
Wavelength range | 360-850 nm |
Data collection | 50-1450 usec, 200 usec increments |
Sensitivity | 0.5 fmol/well Eu chelate |
Luminescence
Detection limit | 10 mol/well alkaline phosphatase 200ul/well |
General photometric performance
Plate formats | 6, 12, 24, 48, 96, 384 wells |
Light source | Xenon flash lamp |
Detector | Photomultiplier (R-3896) |
Read time |
96 wells <15 secs 384 wells <45 secs |
Shaker time |
0-999 seconds |
Temperature range |
Ambient +4C to 45C |
General specs
Dimensions | 8.6"H x 22.8"W x 15"D |
Weight | 35 lbs |
Power source | 100-240 VAC, 3A, 50/60Hz |
Robot compatible |
Yes |
Please don’t hesitate to call or email us if you have any questions.